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Molecular confirmation of BCR-ABL fusion in a chronic myeloid leukemia with a complex translocation involving chromosomes 9, 15, and 22

✍ Scribed by Sugandhi A. Tharapel; Cindy L. Vnencak-Jones; James A. Whitlock; Rajiv Jain

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
274 KB
Volume
4
Category
Article
ISSN
1045-2257

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✦ Synopsis

W e performed molecular studies to resolve the status of BCR and ABL in the bone marrow cells of a CML patient with a Ph chromosome resulting from a complex translocation involving chromosomes 9, 15, and 22. D N A digestion with BomHI, Hindlll, and Bglll, followed by hybridization to a bcr-specific 32P-labeled probe, showed a rearranged banding pattern confirming the involvement of the bcr locus in the translocation. Furthermore, total cellular RNA isolated from the marrow was subjected to reverse transcription into cDNA and amplified by PCR with primers specific for BCR-ABL fusion cDNA. The amplified products obtained from this patient and from a CML patient with the standard t(9;22) were both of the expected length of approximately 3 I7 bp. Genes Chrom Cancer 4:343-345 ( I 992).

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