The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of
Molecular cloning, overexpression and mapping of the slt gene encoding the soluble lytic transglycosylase of Escherichia coli
β Scribed by Betzner, Andreas S. ;Keck, Wolfgang
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 371 KB
- Volume
- 219
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
The gene of the major autolysin of Escherichia coli, the soluble lytic transglycosylase (Slt), was isolated from an expression gene library. The cloned slt gene was used to determine its chromosomal map position adjacent to trp R at 99.7 min on the E. coli linkage map.
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