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Molecular cloning of the human rad gene: gene structure and complete nucleotide sequence

โœ Scribed by James S. Caldwell; Julie S. Moyers; Alessandro Doria; Christine Reynet; Ronald C. Kahn


Book ID
116160028
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
427 KB
Volume
1316
Category
Article
ISSN
0925-4439

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A recombinant lambda phage carrying the recA gene of Rhizobium phaseoli was isolated from a R. phaseoli genomic library by complementation of the Fec- phenotype of the recombinant phage in Escherichia coli. When expressed in E. coli, the cloned recA gene was shown to restore resistance to both UV ir