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Molecular cloning of the 3-phosphoglycerate kinase (PGK) gene fromAspergillus nidulans

✍ Scribed by John M. Clements; Clive F. Roberts


Book ID
104768350
Publisher
Springer-Verlag
Year
1985
Tongue
English
Weight
604 KB
Volume
9
Category
Article
ISSN
0172-8083

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Disruption of the 3β€² phosphoglycerate ki
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We report the isolation of a pgk- mutant strain of Aspergillus nidulans by means of a gene disruption strategy, and demonstrate that the pgk gene is located on chromosome VIII. The pgk- mutant conidiates poorly, will only grow on media supplemented with both a glycolytic and a gluconeogenic carbon s

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✍ Futoshi Nara; Ichiro Watanabe; Nobufusa Serizawa πŸ“‚ Article πŸ“… 1993 πŸ› Springer-Verlag 🌐 English βš– 891 KB

The gene coding for 3-phosphoglycerate kinase (PGK) in ML-236B (compactin)-producing Penicillium citrinum was isolated from the recombinant phage lambda library using the corresponding Aspergillus nidulans pgk gene as a probe. The P. citrinum pgk gene has an open reading frame of 1,254 bp, encoding