Disruption of the 3′ phosphoglycerate kinase (pgk) gene inAspergillus nidulans
✍ Scribed by Stephen J. Streatfield; Clive F. Roberts
- Book ID
- 104735621
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 758 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
✦ Synopsis
We report the isolation of a pgk- mutant strain of Aspergillus nidulans by means of a gene disruption strategy, and demonstrate that the pgk gene is located on chromosome VIII. The pgk- mutant conidiates poorly, will only grow on media supplemented with both a glycolytic and a gluconeogenic carbon source, and is inhibited by hexoses.
📜 SIMILAR VOLUMES
The mammalian genome contains two functional loci for the production of phosphoglycerate kinase, PGK-1, an X-linked gene expressed in all somatic cells, and PGK-2, an autosomal intron-less gene expressed exclusively in late spermatogenesis. A nucleotide substitution from guanine to thymine was recen