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Disruption of the 3′ phosphoglycerate kinase (pgk) gene inAspergillus nidulans

✍ Scribed by Stephen J. Streatfield; Clive F. Roberts


Book ID
104735621
Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
758 KB
Volume
23
Category
Article
ISSN
0172-8083

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✦ Synopsis


We report the isolation of a pgk- mutant strain of Aspergillus nidulans by means of a gene disruption strategy, and demonstrate that the pgk gene is located on chromosome VIII. The pgk- mutant conidiates poorly, will only grow on media supplemented with both a glycolytic and a gluconeogenic carbon source, and is inhibited by hexoses.


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The mammalian genome contains two functional loci for the production of phosphoglycerate kinase, PGK-1, an X-linked gene expressed in all somatic cells, and PGK-2, an autosomal intron-less gene expressed exclusively in late spermatogenesis. A nucleotide substitution from guanine to thymine was recen