Molecular cloning of IL-2Rα, IL-2Rβ, and IL-2Rγ cDNAs from a human oligodendroglioma cell line: Presence of IL-2R mRNAs in the human central nervous system

✍ Scribed by Glen C. Otero; Jean E. Merrill

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 909 KB
• Volume: 14
• Category: Article
• ISSN: 0894-1491
• DOI: 10.1002/glia.440140406

✦ Synopsis

In the present study, we analyzed human adult brain, fetal spinal cord, and an interleukin-2 (IL-2)-responsive human oligodendroglioma subclone, TC620.6A2, for the presence of mRNAs for the a, P, and y chains of the interleukin-2 receptor (IL-2Ra, IL-2RP, and IL-2Ry). IL-2RP mRNA, but not IL-2Ra or IL-2Ry was detectable by Northern blot analysis in adult human brain tissues. Northern blot analysis of TC620.6A2 and human fetal tissues revealed mRNAs of 1.5 kb and 1.3 kb that hybridized to the IL-2Ra cDNA at low to medium stringency. Reverse transcriptase-polymerase chain reaction (RT-PCR) experiments were done on the TC620.6A2 cell line utilizing primers to IL-2Ra, IL-2RP, and IL-2Ry. Southern blot analysis of the TC620.6A2 RT-PCR reactions detected products identical in size to the peripheral blood lymphocyte (PBL) positive controls at high stringency. Several of the TC620.6A2 IL-2Ra, IL-2RP, and IL-2Ry cDNAs were cloned and sequenced. The sequences were found to be identical to the known IL-2R sequences. To our knowledge, these experiments are the first to demonstrate the presence of authentic IL-2R mRNAs in a human oligodendrocyte-like cell line. Demonstration of mRNA for IL-2RP in human adult brain, IL-2Ra in fetal brain, and IL-2Ra, IL-2RP, and IL-2Ry in a malignant neural cell line suggests the possibility of a role for IL-2DL-2R interactions in development and disease. o 1995 Wiley-Liss, Inc.