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Molecular cloning of hepatic 5-aminolevulinate synthase

✍ Scribed by Iain A. BORTHWICK; Gopesh SRIVASTAVA; Andrew A. HOBBS; Byron A. PIROLA; John D. BROOKER; Brian K. MAY; William H. ELLIOTT

Book ID
115122702
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
476 KB
Volume
144
Category
Article
ISSN
1432-1327

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Cloning by genetic complementation and r
Cloning by genetic complementation and restriction mapping of the yeastHEM1gene coding for 5-aminolevulinate synthase
✍ Daniele Urban-Grimal; VΓ©ronique Ribes; Rosine Labbe-Bois πŸ“‚ Article πŸ“… 1984 πŸ› Springer-Verlag 🌐 English βš– 523 KB

We have cloned the structural gene HEMI for 5-aminolevulinate (ALA) synthase from Saccharomyces cerevisiae by transformation and comptementation of a yeast hem1-5 mutant which was previously shown to lack ALA synthase activity (Urban-Grimal and Labbe-Bois 1981) and had no immunodetectable ALA syntha