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Molecular cloning of Drosophila γ-glutamylcysteine synthetase by functional complementation of a yeast mutant

✍ Scribed by Robert D.C. Saunders; Lesley I. McLellan


Book ID
117101470
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
580 KB
Volume
467
Category
Article
ISSN
0014-5793

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A cDNA clone named L21 was isolated from L6 rat muscle cells by complementation of a yeast proline transport mutant. L21 cDNA has 2,268 bp and codes for a peptide of 228 amino acids with four potential transmembrane domains. The amino acid sequence of L21 shows no homology to any known proteins. Exp