Cloning of Arabidopsis thaliana Glutathione Synthetase (GSH2) by Functional Complementation of a Yeast Gsh2 Mutant
β Scribed by Pascaline Ullmann; Laurence Gondet; Serge Potier; Thomas J. Bach
- Book ID
- 115131870
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 949 KB
- Volume
- 236
- Category
- Article
- ISSN
- 1432-1327
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## Summary It is currently thought that most flowering plants lack the capacity to synthesize trehalose, a common disaccharide of bacteria, fungi and invertebrates that appears to play a major role in desiccation tolerance. Attempts have therefore been made to render plants more droughtβresistant b
A yeast null mutant (__erg 3__) defective in __ERG 3__, the gene encoding the Cβ5 sterol desaturase required for ergosterol synthesis was transformed with an __Arabidopsis thaliana__ cDNA library inserted in a yeast vector. Transformants (4Γ10^5^) were screened for cycloheximide (CH) resistance and