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Molecular cloning of a Lupinus polyphyllus cDNA encoding a basic peroxidase isoenzyme of cell suspension cultures

โœ Scribed by Perrey, Ralf; Warskulat, Ulrich; Wink, Michael


Book ID
122700350
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
413 KB
Volume
137
Category
Article
ISSN
0176-1617

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โœ Ralf Perrey; Michael Schneider; Michael Wink ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 119 KB

A cDNA library was constructed from poly(A) + RNA from suspension-cultured cells of Lupinus polyphyllus [ 1 ], which derived from stem tissue [2] and transferred to fresh medium weekly [3]. The cDNA synthesis was carded out using a RNase H approach and Eco RI adaptor strategy [ 1 ]. The cDNA-adaptor