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Molecular cloning of a lupin-specific gene from a cDNA library of suspension-cultured cells ofLupinus polyphyllus

โœ Scribed by Ralf Perrey; Ulrich Warskulat; Michael Wink

Publisher
Springer
Year
1990
Tongue
English
Weight
105 KB
Volume
15
Category
Article
ISSN
0167-4412

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๐Ÿ“œ SIMILAR VOLUMES

Tissue-specific expression of a cDNA clo
Tissue-specific expression of a cDNA clone from cell suspension cultures ofLupinus polyphyllus
โœ Ralf Perrey; Michael Schneider; Michael Wink ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 119 KB

A cDNA library was constructed from poly(A) + RNA from suspension-cultured cells of Lupinus polyphyllus [ 1 ], which derived from stem tissue [2] and transferred to fresh medium weekly [3]. The cDNA synthesis was carded out using a RNase H approach and Eco RI adaptor strategy [ 1 ]. The cDNA-adaptor