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Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme

โœ Scribed by Hiroshi OHNISHI; Hiroki KITAMURA; Takashi MINOWA; Hiroshi SAKAI; Takahisa OHTA


Book ID
115129429
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
713 KB
Volume
207
Category
Article
ISSN
1432-1327

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Molecular cloning and characterization o
โœ Pretorius, Isak S. ;Chow, Thomas ;Modena, Daniela ;Marmur, Julius ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 761 KB

The Saccharomyces diastaticus structural gene STA2, encoding an exracellular glucoamylase (1,4-alpha-D-glucan glycohydrolase, EC 3.2.1.3.), has been cloned by complementation of a stao strain. A genomic library was initially constructed from a STA2 yeast strain in the yeast Escherichia coli shuttle