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Cloning and sequencing of a Clostridium perfringens sialidase gene

โœ Scribed by Peter Roggentin; Bernd Rothe; Friedrich Lottspeich; Roland Schauer

Book ID
115920370
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
346 KB
Volume
238
Category
Article
ISSN
0014-5793

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The sialidase gene from Clostridium sept
The sialidase gene from Clostridium septicum: cloning, sequencing, expression in Escherichia coli and identification of conserved sequences in sialidases and other proteins
โœ Rothe, Beate ;Rothe, Bernd ;Roggentin, Peter ;Schauer, Roland ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 840 KB

An oligonucleotide mixture corresponding to the codons for conserved and repeated amino acid sequences of bacterial sialidases (Roggentin et al. 1989) was used to clone a 4.3 kb PstI restriction fragment of Clostridium septicum DNA in Escherichia coli. The complete nucleotide sequence of the sialida