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Molecular cloning and expression of the genes encoding the Escherichia coli K4 capsular polysaccharide, a fructose-substituted chondroitin

โœ Scribed by Drake, C


Book ID
122114123
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
183 KB
Volume
66
Category
Article
ISSN
0378-1097

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โœ Goss, Thomas J. ;Datta, Prasanta ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 944 KB

The biodegradative threonine dehydratase gene (tdc) of Escherichia coli was cloned by isolating a dehydratase negative mutant after Tn5 mutagenesis, cloning the tdc::Tn5 DNA into pBR322 and then replacing the Tn5 element on the plasmid in vivo. Subcloning and nucleotide sequence data revealed two di