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Cloning and expression of the Arthrobacter globiformis KZT1 fcbA gene encoding dehalogenase (4-chlorobenzoate-4-hydroxylase) in Escherichia coli

โœ Scribed by Tamara V. Tsoi; Gennady M. Zaitsev; Elena G. Plotnikova; Irina A. Kosheleva; Alexander M. Boronin


Book ID
109317195
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
321 KB
Volume
81
Category
Article
ISSN
0378-1097

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Plasmid pAO1 of Arthrobacter oxidans enc
โœ Brandsch, Roderich ;Faller, Waltraud ;Schneider, Klaus ๐Ÿ“‚ Article ๐Ÿ“… 1986 ๐Ÿ› Springer ๐ŸŒ English โš– 815 KB

The 160 kb plasmid pAO1 from Arthrobacter oxidans (Brandsch and Decker 1984) was subcloned in Escherichia coli with the aid of the plasmid vectors pUR222 and pBR322. Screening of the recombinant clones for enzyme activity revealed that the flavoenzyme 6-hydroxy-D-nicotine oxidase (6-HDNO), one of th