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Molecular cloning and expression of a δ-opioid recetpor from rat brain

✍ Scribed by M. E. Abood; M. A. Noel; J. S. Farnsworth; Q. Tao

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
496 KB
Volume
37
Category
Article
ISSN
0360-4012

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✦ Synopsis

We have isolated and characterized a rat 6-opioid receptor. The deduced amino acid sequence (372 aa) closely resembles the murine 6-opioid receptor, DOR-1. In fact, 97% of the amino acid residues are conserved between the two species, while 93% of the nucleic acid residues are identical. A 6 kb mRNA was detected in rat cortex using rat DOR-1 as a probe. When expressed in COS cells, the clone shows highaffinity opioid binding with selectivity for 6-opioids. The rat 6-opioid receptor cDNA clone will be a useful tool for studying the function of 6-opioid receptor in rats.

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