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Molecular cloning and expression of a Cu/Zn-Containing superoxide dismutase fromThellungiella halophila

โœ Scribed by Xiaojing Xu; Yijun Zhou; Shanjun Wei; Dongtao Ren; Min Yang; Huahu Bu; Mingming Kang; Junli Wang; Jinchao Feng


Publisher
Korean Society for Molecular and Cellular Biology
Year
2009
Tongue
English
Weight
712 KB
Volume
27
Category
Article
ISSN
1016-8478

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A procedure has been developed to distinguish between the two forms of eukaryotic superoxide dismutases using a common activity assay. Treatment of cellular fractions with 2% sodium dodecyl sulfate at 37ยฐC for 30 min selectively inactivates the mitochondrial, manganese-containing variant without aff