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A method for distinguishing Cu,Zn- and Mn-containing superoxide dismutases

✍ Scribed by Bruce L. Geller; Dennis R. Winge

Publisher: Elsevier Science
Year: 1983
Tongue: English
Weight: 566 KB
Volume: 128
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(83)90348-2

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✦ Synopsis

A procedure has been developed to distinguish between the two forms of eukaryotic superoxide dismutases using a common activity assay. Treatment of cellular fractions with 2% sodium dodecyl sulfate at 37°C for 30 min selectively inactivates the mitochondrial, manganese-containing variant without affecting the cytosolic copper, zinc-superoxide dismutase. After removing excess sodium dodecyl sulfate by precipitation with potassium chloride, the supernate is assayed using the xanthine oxidase-cytochrome c method.

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