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Molecular cloning and enzymatic characterization of a Trichoderma reesei 1,2-α-d-mannosidase

✍ Scribed by Marleen Maras; Nico Callewaert; Kathleen Piens; Marc Claeyssens; Wim Martinet; Sylviane Dewaele; Hans Contreras; Isabelle Dewerte; Merja Penttilä; Roland Contreras

Book ID
119525284
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
243 KB
Volume
77
Category
Article
ISSN
0168-1656

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✍ Yutaka Maruyama; Tasuku Nakajima; Eiji Ichishima 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 500 KB

A 1,2-&D-mannosidase was purified to homogeneity from the culture supernatant of Bacillus sp. M-90, which was isolated from soil by enrichment culture on baker's yeast mannan. The purified enzyme had M, 380000 Da, and was comprised of two apparently identical 190000 Da subunits. It had a neutral opt