After uptake of microbial ferrisiderophores, iron is assumed to be released by reduction. Two ferrisiderophore-reductase activities were identified in Escherichia coli K-12. They differed in cellular location, susceptibility to amytal, and competition between oxygen and ferrichrome-iron(III) reducti
Molecular cloning and characterization ofEscherichia coliK12ygjGgene
โ Scribed by Natalya N Samsonova; Sergey V Smirnov; Irina B Altman; Leonid R Ptitsyn
- Book ID
- 115005424
- Publisher
- BioMed Central
- Year
- 2003
- Tongue
- English
- Weight
- 303 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1471-2180
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Cells of Escherichia coli K-12 could grow aerobically at an iron concentration as low as 0.05 laM without any of the known iron ionophores present. The growth rate increased between 0.05 and 2 gM iron. Supplementation with the iron ligands ferrichrome and citrate resulted in optimal growth already a
ColE1amp plasmids carrying the entire bio gene cluster were constructed in vitro using ColE1amp as the cloning vehicle and a lambda transducing phage, lambdaatt2, as the source of bio DNA. Restriction endonuclease EcoRI digests of ColE1amp and lambdaatt2 DNA were joined by polynucleotide ligase and