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Iron uptake and iron limited growth ofEscherichia coliK-12

✍ Scribed by Anton Hartmann; Volkmar Braun

Book ID
104764846
Publisher
Springer
Year
1981
Tongue
English
Weight
450 KB
Volume
130
Category
Article
ISSN
0302-8933

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✦ Synopsis

Cells of Escherichia coli K-12 could grow aerobically at an iron concentration as low as 0.05 laM without any of the known iron ionophores present. The growth rate increased between 0.05 and 2 gM iron. Supplementation with the iron ligands ferrichrome and citrate resulted in optimal growth already at 0.05 laM iron. Under certain conditions iron uptake preceded growth of cells by more than an hour. During logarithmic growth the rate of iron uptake matched the growth rate. The radioactive tracer method revealed a cellular iron content of 4 nmol/mg dry weight.

After consumption of the iron in the medium cells continued to grow with high rate for 1 -2 generations. The iron uptake activity was increased during iron starvation.

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Iron uptake in pseudorevertants ofEscher
Iron uptake in pseudorevertants ofEscherichia coliK-12 mutants with multiple defects in the enterochelin system
✍ Carol L. Pickett; Charles F. Earhart πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 717 KB

Iron uptake in pseudorevertants of Escherichia coli K-12 strains which lack the ability to synthesize enterochelin, 2,3 dihydroxybenzoate, and the ferrienterochelin receptor protein was characterized. In four independent pseudorevertants, the suppressor mutations which permitted growth in iron-poor