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Molecular cloning and characterization of the purE operon of Escherichia coli

✍ Scribed by Kamholz, John; Keyhani, Jacqueline; Gots, Joseph S.

Book ID
122555407
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
706 KB
Volume
44
Category
Article
ISSN
0378-1119

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Using methods of in vitro recombination we constructed hybrid plasmids that can suppress the increased methylmethane sulfonate sensitivity caused by alkB mutation. Since the cloned DNA fragment was mapped at 47 rain on the Escherichia coli K12 genetic map, an area where the alkB gene is located, we