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Cloning and transfer of sucrase operon in Escherichia coli

✍ Scribed by Marian Mačor; Ján Turňa; Jozef Grones


Book ID
116107470
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
454 KB
Volume
26
Category
Article
ISSN
0167-7012

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The glutamine permease operon encoding the high-affinity transport system of glutamine in Escherichia coli could be cloned in one of the mini F plasmids, but not in pBR322 or pACYC184, by selection for restoration of the Gln+ phenotype, the ability to utilize glutamine as a sole carbon source. We de