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Molecular cloning and characterization of the alcohol dehydrogenase ADH1 gene ofCandida utilisATCC 9950

✍ Scribed by Yong-Cheol Park; Na-Rae Yun; Ka-Yiu San; George N. Bennett

Publisher
Springer-Verlag
Year
2006
Tongue
English
Weight
436 KB
Volume
33
Category
Article
ISSN
1476-5535

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Two Pichia stipitis ADH genes (PsADH1 and PsADH2) were isolated by complementation of a Saccharomyces cerevisiae Adh -mutant. The genes enabled the transformants to grow in the presence of antimycin A on glucose, to use ethanol as sole carbon source and made them sensitive to allylalcohol. The sequ