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Molecular cloning and characterization of a ras p21-like GTP-binding protein (24KG) from rat liver

✍ Scribed by Kazuhiro Sakurada; Kazuhisa Uchida; Kazuo Yamaguchi; Kazuo Aisaka; Seiga Ito; Toshihiko Ohmori; Yoshifumi Takeyama; Takashi Ueda; Yuichi Hori; Harumasa Ohyanagi; Yoichi Saitoh; Kozo Kaibuchi; Yoshimi Takai


Book ID
115764019
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
729 KB
Volume
177
Category
Article
ISSN
0006-291X

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✍ Colette Jako; Bernard Teyssendier Serve πŸ“‚ Article πŸ“… 1996 πŸ› Springer 🌐 English βš– 388 KB

The cloning of small GTP-binding proteins from Petunia hybrida was performed using a PCR-based strategy. Degenerate primers were designed from the DTAGQE and FMETSA consensus sequences. Three different cDNAs were amplified. The deduced polypeptide sequences PhPCRGP1 and PhPCRGP2 were homologous to R