๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Molecular cloning and analysis of chromosome band 11q23 involved in leukaemia-associated translocations

โœ Scribed by Soma Das; Lyndal Kearney; Mark Bower; Tracy Chaplin; John H. Riley; Rakesh Anand; Bryan D. Young

Book ID
102845429
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
821 KB
Volume
5
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

โœฆ Synopsis

Three overlapping yeast artificial chromosomes (YACs) spanning a 780 kb region of DNA around the CD3 locus on chromosome I I have been isolated and characterised. The individual cloned regions have been mapped by in situ hybridisation to chromosome band I lq23, and a restriction enzyme map of this region has been constructed. The positions of these clones in relation to a series of leukaemia-associated chromosomal translocations has also been determined. It was concluded that. although two clones lay entirely proximal to the breakpoints examined, the third clone ( I 3HH4) encompassed the breakpoints for the translocations t(4;I I), t(6;I I), and t(9;I I). The t(9;I I ) was observed in an acute myeloid leukaemia in a patient previously treated for an unrelated malignancy. It would thus appear that the breakpoints at chromosome band I lq23 occurring in therapy-related leukaemias are in the same region as those found in adult and childhood acute leukaemias and may result from a common underlying mechanism. Genes Chrorn Cancer 5244-25 I ( I 992). @ 1992 WiIey-Lits, Inc.

๐Ÿ“œ SIMILAR VOLUMES

Molecular analysis of the rearranged gen
Molecular analysis of the rearranged genome and chimeric mRNAs caused by the t(6;11)(q27;q23) chromosome translocation involving MLL in an infant acute monocytic leukemia
โœ Yukihiro Akao; Masaharu Isobe ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 158 KB ๐Ÿ‘ 2 views

Chromosomal analysis of acute monocytic leukemia cells in a female infant revealed a t(6;11)(q27;q23) translocation. Southern blot analysis with a cDNA probe of the MLL gene at chromosome band 11q23 indicated that the breakpoint was in an 8.3-kb BamHI fragment that contained exons 5-11 of the MLL ge

Molecular analysis of a new translocatio
Molecular analysis of a new translocation, t(X;I4)(q28;qII), in premalignancy and in leukaemia associated with ataxia telangiectasia
โœ J. Thick; P. D. Sherrington; P. Fisch; A. M. R. Taylor; T. H. Rabbitts ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 471 KB ๐Ÿ‘ 1 views

The disease ataxia telangiectasia (A-T) is a multifaceted disorder in which patients have an increased chance of developing a T-cell leukaemia, often with abnormalities of chromosome 14, but sometimes with rare translocations, like t(X; 14)(q28;q I I). We describe the cloning of the breakpoint of on