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Molecular and functional characterization of the Azorhizobium caulinodans ORS571 hydrogenase gene cluster

✍ Scribed by Cecilia Baginsky; Jose-Manuel Palacios; Juan Imperial; Tomás Ruiz-Argüeso; Belén Brito

Book ID
109328013
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
400 KB
Volume
237
Category
Article
ISSN
0378-1097

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## Abstract An R‐prime plasmid (pJB3JI‐18) carrying a 10 kb region of __Azorhizobium caulinodans__ ORS571 DNA which codes for uptake hydrogenase activity was isolated from a Km^8^ derivative of R68.45. R‐prime plasmids were obtained by transferring R68.45 to ORS571, retransferring it to __E. coli__