The fast growing strain, Azorhizobium caulinodans ORS571, isolated from stem nodules of the tropical legume Sesbania rostrata, can grow in the free-living state at the expense of molecular nitrogen. Five point mutants impaired in nitrogen fixation in the free-living state have been complemented by a
Physical and biochemical characterization of a competition and nodulation defective mutant of Azorhizobium caulinodans ORS571
β Scribed by Dr. L. Kishore; K. Natarajan; C. R. Babu
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 744 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0233-111X
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The nucleotide sequence of a 4.1 kb DNA fragment containing the fixABC region of Azorhizobium caulinodans was established. The three gene products were very similar to the corresponding polypeptides of Rhizobium meliloti. The C-terminal domains of both fixB products displayed a high degree of simila
## Abstract A mutant (A204) of Chinese hamster ovary cells (CHOβK1), which is deficient in dihydroorotate (DHO) dehydrogenase (E.C. 1.3.3.1) activity, has been isolated by a replica plating procedure. The mutant does not show a requirement for exogenously added pyrimidines. Examination of intact ce