Molecular analysis of T-lymphocyte HPRT- mutations in individuals exposed to ionizing radiation in Goiânia, Brazil

✍ Scribed by Adonis Skandalis; Aparecido D. Da Cruz; John Curry; Axel Nohturfft; Maria P. Curado; Barry W. Glickman

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 94 KB
• Volume: 29
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)29:2<107::aid-em1>3.0.co;2-b

✦ Synopsis

We have characterized 54 HPRT 0 point mutations sertions, complex mutations, and losses of exon sein T-lymphocytes from 17 individuals exposed to quences from the mRNA. The relative frequency of ionizing radiation of 137 Cs in Goia ˆnia, Brazil and the different mutation types was similar in the two compared this spectrum to that of 30 HPRT 0 mutants studied groups. However, in our study the distribufrom 9 unexposed Brazilian controls. The average tion of events within the hprt coding sequence internal exposure of the exposed group was 205 seemed to cluster at the same regions of the gene. mCi, and the average external exposure was 1.7 These observations imply that the hprt gene does Gy. The average HPRT 0 mutant frequency for the not present a homogeneous target to radiation muexposed group was 13.3 1 10 05 , approximately tagenesis, and perhaps this class of information a 10-fold increase over the mutant frequency of the may be used to detect radiation exposure in human unexposed controls, which was 1.56 1 10 05 . The populations. Environ. Mol. Mutagen.