Monitoring hprt mutant frequency over time in T-lymphocytes of people accidentally exposed to high doses of ionizing radiation
✍ Scribed by Aparecido D. da Cruz; John Curry; Maria P. Curado; Barry W. Glickman
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 911 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0893-6692
No coin nor oath required. For personal study only.
✦ Synopsis
Modern technologies have provided the opportu-of the control group was 2.5 (20.2) + ln10-6. nity to monitor mutations in people in vivo. The sub The exposed group had a significantly increased jects of this study were accidentally exposed to mutant frequency; the mean In MF (?SE) were 3.3 '37Cesium in a radiological accident that occurred (20.3) + ln10-6, 2.8 (50.2) + In10 ', and 2.3 in September 1987 in GoiBnia, Brazil, during (20.2) + ln10-6, in the years 1990-1992 rewhich more than 150 people received doses spectively. Based on the decline of mutant fregreater than 0.1 Gy and as high as 7 Gy. The quency and using Buckton's models [Buckton et objective of this study was to determine how long al. (1 967): Nature 2 14:470-4731, we demonthe hprt mutant Tcells in the peripheral blood con-strated that mutant T-cells have a short-term memtribute to mutant frequency by examining the time-ory with a half-life of 2.1 years. This relatively course of the T-lymphocyte response to ionizing ra-short half-life limits the effective use of the hprt diation. This report describes the results obtained assay as the method of choice to monitor past over a period of 2.3 to 4.5 years subsequent to the exposure. The data also demonstrate a positive accident, from 1 1 subjects with doses ranging from correlation with age, and an inverse correlation 1 to 7 Gy, and from nine control subjects selected with plating efficiency. from the same population. The mean In MF (2SE)