Molecular analysis of mutations in T-lymphocytes from experienced Soviet cosmonauts

✍ Scribed by M. Khaidakov; D. Young; H. Erfle; A. Mortimer; Y. Voronkov; B. W. Glickman

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 85 KB
• Volume: 30
• Category: Article
• ISSN: 0893-6692
• DOI: 10.1002/(sici)1098-2280(1997)30:1<21::aid-em4>3.0.co;2-f

✦ Synopsis

Somatic mutation in five cosmonauts who have com-analyzed by the sequencing of exon(s)-containing pleted spaceflights of 7 to 365 days was analyzed fragments amplified from genomic DNA. The mutausing the clonal HPRT assay. The doses received in tional spectrum recovered from the cosmonauts difspace by the cosmonauts ranged from 4 to 127 fered substantially from that of unexposed healthy mGy. hprt mutant frequencies were 2.4-5.0-fold subjects (P Å 0.042), and exhibited an increased higher than age-corrected values established for incidence of splicing errors, frameshifts, and comhealthy, unexposed subjects in western countries plex mutations. Higher frequencies of contribution [Tates et al. (1991): Mutat Res 253:199 -213; of AT r GC transitions and GC r TA transversions Branda et al. (1993): Mutat Res 285:267 -279] were also observed. The increased mutant frequenand 2-to 3-fold higher than those determined for cies and observed shifts in mutational spectra likely unexposed individuals residing in Russia [Jones et indicate a combination of potential influences, inal. (1995): Mutat Res 338:129-139]. A total of cluding environment, lifestyle, and occupational 107 collected mutant clones were analyzed by mul-exposures. Further elucidation of these potential intiplex PCR. No excess of deletions was detected fluences will require a more extensive study involvand their frequency did not correlate with either ing the general population sharing similar enviaccumulated dose or the age of the cosmonauts. ronment, cosmonauts in training and cosmonauts In 62 mutants cDNA was isolated by RT-PCR and participating in space flights. Environ. Mol. Mutasequenced. Those with splicing errors, as well as gen . 30:21 -30, 1997 ᭧ 1997 Wiley-Liss, Inc. the mutants that did not produce cDNA, were further