Modulation of the regioselectivity of a Bacillus α-galactosidase by directed evolution
✍ Scribed by Michel Dion; Audrey Nisole; Petra Spangenberg; Corinne André; Alexandra Glottin-Fleury; Ralf Mattes; Charles Tellier; Claude Rabiller
- Book ID
- 110313281
- Publisher
- Springer US
- Year
- 2001
- Tongue
- English
- Weight
- 174 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1573-4986
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Incubation of a solution containing u-galactose and a high concentration of sucrose in the presence of a-D-galactosidase from M. vinacea afforded raffinose and planteose in a ratio of -3:2. On the other hand, circulation of a solution of D-g&iCtOSe and sucrose through a reaction system consisting of
## Abstract The α‐galactosidase (AGA) from __Bifidobacterium adolescentis__ DSM 20083 has a high transglycosylation activity. The optimal conditions for this activity are pH 8, and 37°C. At high melibiose concentration (600 mM), approximately 64% of the enzyme–substrate encounters resulted in trans