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Modulation of the Expression of Liver-Specific Functions in Novel Human Hepatocyte Lines Cultured in a Collagen Gel Sandwich Configuration

โœ Scribed by Yumi Kono; Eve A. Roberts


Book ID
115578217
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
525 KB
Volume
220
Category
Article
ISSN
0006-291X

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A serum-free culture system for primary hepatocytes which maintains stable high-level hepatocyte function for prolonged periods in culture has been developed. Isolated rat primary hepatocytes were cultured in serum-free media between two layers of gelled collagen in a sandwich configuration which re