The importance of proline on long-term hepatocyte function in a collagen gel sandwich configuration: Regulation of protein secretion
✍ Scribed by Jaegwan Lee; Jeffrey R. Morgan; Ronald G. Tompkins; Martin L. Yarmush
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 743 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
A serum-free culture system for primary hepatocytes which maintains stable high-level hepatocyte function for prolonged periods in culture has been developed. Isolated rat primary hepatocytes were cultured in serum-free media between two layers of gelled collagen in a sandwich configuration which reinstates the cellular polarity necessary for long-term function in vitro. These serum-free hepatocyte cultures maintained near physiological rates of albumin and transferrin secretion for a minimum of 26 days in culture. L-Proline was shown to be critical for both the approach to steady state and maximal level of protein secretion. Analysis of dose-response data gave K, values of 2.9 and 1.7 pg/mL for albumin and transferrin secretion, respectively.