Modulation of membrane phenotype, matrix adhesion and microinvasiveness of metastatic tumour cells by HUdR

✍ Scribed by J. Timár; G. Pogány; M. Balázs; J. Szöllösi; A. Ladányi; J. Oláh; F. Timár; K. Lapis; A. Jeney

• Book ID: 102789312
• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 865 KB
• Volume: 8
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/cbf.290080405

✦ Synopsis

Abstract

The effect of HUdR, proved to be anti‐metastatic in vivo, was studied in vitro on cell proliferation, nucleoside uptake, membrane fluidity, expression of galactosylated glycans and proteoglycans in metastatic HM tumour cells. The observed increase in membrane fluidity and the suppression of nucleoside transport were early events of the HUdR action followed by decrease of galactosylated glycan and HSPG expression. However, these changes did not influence the proliferation capacity of the cells at the concentrations studied. As a consequence of the membrane alterations a reduced adhesiveness and spreading on extracellular matrix components was detected. In addition, the HUdR treated HM cells showed reduced capacity to invade fibroblast monolayers in vitro. Based on these observations, HUdR could be the prototype of new anti‐metastatic agents acting at the level of tumour–host interaction.