✦ LIBER ✦

Influence of shear stress on tumor-cell adhesion to endothelial-cell extracellular matrix and its modulation by fibronectin

✍ Scribed by Eva Bastida; Lourdes Almirall; M. Cruz Bertomeu; Antonio Ordinas

Publisher: John Wiley and Sons
Year: 1989
Tongue: French
Weight: 533 KB
Volume: 43
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910430635

No coin nor oath required. For personal study only.

✦ Synopsis

The interaction of malignant cells with blood-vessel endothelial cells and their underlying basement membrane is an important step in the development of secondary metastases. We investigated the interactions of highly metastatic human tumor cells, the A-549 adenocarcinoma of the lung, with cultured endothelial cells (EC) and their extracellular matrix (ECM). We studied the adhesion patterns of the A-549 tumor cells to EC and ECM under static and flow conditions. Our results provide evidence that tumor-cell adhesion depends not only on the characteristics of the tumor cells themselves, but also on the properties of the EC and ECM. Our results also indicate that tumor-cell adhesion to ECM is shear-ratedependent, and that it is partially modulated by fibronectin. Moreover, our results suggest that the arg-gly-asp (RGD) common adhesion receptor site is also involved in the adhesion of the A-549 cells to EC and ECM.

📜 SIMILAR VOLUMES

Heterogeneity for integrin expression an

Heterogeneity for integrin expression and cytokine-mediated VLA modulation can influence the adhesion of human melanoma cells to extracellular matrix proteins

✍ Roberta Mortarini; Andrea Anichini; Giorgio Parmiani 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 French ⚖ 966 KB

Expression of a and p subunits of VLA and VNR integrins was analyzed by cytofluorimetric analysis on 6 different human primary and metastatic melanoma cell cultures. Marked inter-tumor heterogeneity was observed, and expression of VLA-a I, VLA-a2 and VLA-a6 was lower on primary melanomas than on met

Modulation of functional responses of en

Modulation of functional responses of endothelial cells linked to angiogenesis and inflammation by shear stress: Differential effects of the mechanotransducer CD31

✍ Katie Glen; N. Thin Luu; Ewan Ross; Chris D. Buckley; G. Ed Rainger; Stuart Eggi 📂 Article 📅 2012 🏛 John Wiley and Sons 🌐 English ⚖ 721 KB

## Abstract We investigated the roles of the “mechanotransducer” CD31 in the effects of shear stress on endothelial gene expression and functional responses relevant to angiogenesis and inflammation. Human or murine endothelial cells (hEC or mEC) were exposed to different levels of shear stress, wh