Modulation of a Cell Surface Glycoprotein in Yeast: Acid Phosphatase
โ Scribed by M. ERNST SCHWEINGRUBER; ANNE-MARIE SCHWEINGRUBER
- Book ID
- 115110882
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 256 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0301-4681
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๐ SIMILAR VOLUMES
An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz
In the conrse of experiments with liviiig yeast cells, it mas found tliat adenmine triyliosphate (ATP) added to the mecliuin was hydrolyzed to inorganic phosphate plus aderiylic acid. Tlie quantitative recovery of the 1woclncts of lipclrolysis in the inediuxn suggested tliat the phospliate-splittiii