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Modified oligoribonucleotides as site-specific probes of RNA structure and function

✍ Scribed by David J. Earnshaw; Michael J. Gait

Publisher: Wiley (John Wiley & Sons)
Year: 1998
Tongue: English
Weight: 581 KB
Volume: 48
Category: Article
ISSN: 0006-3525
DOI: 10.1002/(sici)1097-0282(1998)48:1<39::aid-bip5>3.0.co;2-a

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✦ Synopsis

Modified nucleotides can be incorporated site specifically into RNA by the use of total chemical synthesis as well as by use of a variety of recombinant RNA techniques. The range of nucleotide analogues includes modifications to base, sugar, and phosphate for structure-function analysis and for cross-linking studies as well as to answer specific mechanistic questions in RNA catalysis. We describe how RNA containing site-specific modifications are prepared, concentrating in particular on routes involving chemically synthesized oligoribonucleotides, and give examples of their application in studies of the hammerhead and hairpin ribozymes.

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