A simple, rapid, and sensitive fluorometric assay for measuring the activity of tyrosine hydroxylase is described. The enzyme activity is detected by converting tyrosine to 3,4-dihydroxyphenylalanine (dopa), which is then subjected to conversion to the highly fluorescent product by the trihydroxyind
Modification of the tyrosine hydroxylase assay
✍ Scribed by Pauline Lerner; Peter Nosé; Matthew M. Ames; Walter Lovenberg
- Book ID
- 105016832
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 587 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0364-3190
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Tyrosine hydroxylase can be measured by release of tritiated water from labeled tyrosine, and the assay method has now been modified to allow recovery of 3H2O from the reaction mixture in a much more rapid and less tedious manner than previously possible. In the new method, the tyrosine hydroxylase
The present report describes a highly sensitive method for measuring tyrosine hydroxylase based on the enzymic decarboxylation of 14Ccarboxyl labeled n-dihydroxyphenylalanine (dopa) formed from car-boxy1 labeled n-tyrosine. Radiochemical methods for the determination of tyrosine hydroxylase activity