Modification of the mouse mammary gland organ culture technique
β Scribed by R. G. Mehta; W. L. Cerny; M. J. Madigan; R. C. Moon
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 403 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1573-0603
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Apoptosis was measured in mammary glands during whole organ culture, to determine whether regression resulting from hormone withdrawal results in epithelial cell death as in vivo involution. Glands were evaluated for morphology and DNA degradation prior to whole organ culture, after lobulo-alveolar
## Abstract DNA synthesis (LI) and mitotic activity (MI) were studied at different times of the day in the developing mammary gland end bud of the postnatal mouse. Both LI and MI displayed a clear diurnal variation. Animals studied at the middle of the 12βhr dark cycle showed a LI 2.1β2.6 times tha