Modification and Evaluation of a Reverse Phase High Performance Liquid Chromatographic Method for Amino Acid Analysis
โ Scribed by T. A. KAN; W. F. SHIPE
- Book ID
- 108805599
- Publisher
- Institute of Food Technologists
- Year
- 1982
- Tongue
- English
- Weight
- 275 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0022-1147
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๐ SIMILAR VOLUMES
High-performance liquid chromatography was used to separate physiological amino acids in perchloric acid supernatants of blood samples. Precolumn derivatization with phenyl isothiocyanate was carried out, starting with 20 microliters of supernatant; 2-10 microliters were injected into a 30-cm Pico T
All 24 dansyl amino acids were separated by reverse-phase high-performance liquid chromatography on Develosil C8-5, using a linear gradient made from Tris-HCl buffer (pH 7.75) and methanol. A linear relationship between the amount of sample and peak area was found over the range of 6 to 300 ng (0.02