Modern Fluorescence Spectroscopy

✍ Scribed by John W. Lyons, Patrick T. Hardesty, Charles S. Baer (auth.), E. L. Wehry (eds.)

• Publisher: Springer US
• Year: 1981
• Tongue: English
• Leaves: 369
• Series: Modern Analytical Chemistry
• Edition: 1
• Category: Library

✦ Synopsis

Since the appearance of the first two volumes of Modern Fluorescence Spectroscopy in 1976, important advances continue to be made in both the techniques and applications of molecular luminescence. In terms of "hardware," it is only recently that the application of laser excitation to molecular fluorometry has become feasible under conditions that are analy­ tically realistic. The improvements that can be effected in sensitivity, analy­ tical selectivity, and ability to handle "difficult" samples by laser fluorometry have only begun to be exploited. Likewise, time-resolved fluorometry has received widespread use in fundamental studies (a sizable number of which deal with biological systems), but has as of yet received relatively little analytical utilization. The use of electronic array detectors offers the promise of obtaining luminescence spectra more rapidly, and perhaps ultimately with greater sensitivity, than is possible by the use of scanning instruments equipped with conventional detectors. The increasing capabilities of microcomputers and the increasing sophistication of "smart" spectroscopic instrumentation signify that much more efficient acquisition and use can now be achieved of the information contained in the "excitation-emission matrix" inherent in the luminescence phenomenon.

✦ Table of Contents

Front Matter....Pages i-xx
Structural Interpretation of Fluorescence Spectra by Automated File Searching....Pages 1-33
Fluorescence Detection in Liquid and Gas Chromatography....Pages 35-94
Reaction Rate Methods in Fluorescence Analysis....Pages 95-142
Principles and Practice of Fluoroimmunoassay Procedures....Pages 143-191
Fluorometric Studies of Biologically Important Molecular Complexes....Pages 193-249
Fluorometric Quantification of Specific Chemical Species in Single Cells....Pages 251-293
Microspectrofluorometric Procedures and Their Applications in Biological Systems....Pages 295-346
Back Matter....Pages 347-354

✦ Subjects

Analytical Chemistry