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Modeling the electrophoresis and transport of peptides: The effective sphere model and complex formation

โœ Scribed by Stuart A. Allison; Hongxia Pei; Michelle Allen; Jocelyn Brown; Chang-Il Kim; Yang Zhen

Book ID
102446084
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
209 KB
Volume
33
Category
Article
ISSN
1615-9306

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โœฆ Synopsis

Abstract

Modeling the electrophoretic mobility of peptides is examined in this study using a โ€œcoarse grainedโ€ bead model, or B model for short 8 and also a simpler โ€œeffective sphereโ€ (ES) model. A comparison between the B and ES models is carried out for peptide models covering a broad range of ionic strength, peptide charge, and peptide length. At ionic strengths lower than approximately 0.013โ€‰M, the B and ES models agree to within a few percent. The ES model is much simpler than the B model and is of particular value in certain applications such as complex formation between peptide and other species in the BGE. The mobility behavior of oligoglycine in a borate buffer at high pH can be accounted for when complex formation is included in modeling.

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