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Modeling the electrophoresis of highly charged peptides: Application to oligolysines

✍ Scribed by Hengfu Wu; Stuart A. Allison; Catherine Perrin; Herve Cottet

Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
196 KB
Volume
35
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

The β€œcoarse‐grained” bead modeling methodology, BMM, is generalized to treat electrostatics at the level of the nonlinear Poisson–Boltzmann equation. This improvement makes it more applicable to the important class of highly charged macroions and highly charged peptides in particular. In the present study, the new nonlinear Poisson–Boltzmann, NLPB‐BMM procedure is applied to the free solution electrophoretic mobility of low molecular mass oligolysines (degree of polymerization 1–8) in lithium phosphate buffer at pH 2.5. The ionic strength is varied from 0.01 to 0.10 M) and the temperature is varied from 25 to 50Β°C. In order to obtain quantitative agreement between modeling and experiment, a small amount of specific phosphate binding must be included in modeling. This binding is predicted to increase with increasing temperature and ionic strength.

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