Modeling gradient elution of proteins in ion-exchange chromatography
โ Scribed by Stuart R. Gallant; Suresh Vunnum; Steven M. Cramer
- Publisher
- American Institute of Chemical Engineers
- Year
- 1996
- Tongue
- English
- Weight
- 992 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0001-1541
No coin nor oath required. For personal study only.
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## Abstract Protein elution curves in ion exchange chromatography (IEC) were simulated with a rate model. Three pure proteins and their mixture were used (ฮฑโlactalbumin, BSA, and conalbumin) under different operational conditions. The anionic matrix QโSepharose FF was used packed in a 1โmL column.
## Abstract Gradient elution in ion chromatography (IC) offers several advantages: total analysis time can be significantly reduced, overall resolution of a mixture can be increased, peak shape can be improved (less tailing) and effective sensitivity can be increased (because there is little variat
In ideal displacement chromatography systems with infinite mass-transfer kinetics , ยฎarious solutes are separated by sharp discontinuities. In real systems, howeยฎer, the shocks are eroded into shock layers because of the finite rates of mass transfer. The thickness of these shock layers, which can r