๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Model for the influence of mass transport on enzymatic hydrolysis of polysaccharides

โœ Scribed by Dr.-Ing. Torsten Heitmann; Dr. Edda Wenzig; Prof. Dr.-Ing. Alfons Mersmann

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
758 KB
Volume
20
Category
Article
ISSN
0930-7516

No coin nor oath required. For personal study only.

โœฆ Synopsis

In this paper enzymatic hydrolysis reactions have been considered as integration-limited reactions analogous to crystal growth. Based on the mathematical formulation of diffusion of polysaccharide substrate molecules to binding sites of the enzyme, integration kinetics were derived and compared to measured data. Significant is the measured and calculated decrease of the ratio of the specific activity and the Michaelis-Menten parameter with increasing molar mass of the enzymes with an exponent more than unity.

๐Ÿ“œ SIMILAR VOLUMES

The combined use of enzymatic hydrolysis
The combined use of enzymatic hydrolysis and fast atom bombardment mass spectrometry for peptide sequencing
โœ R. Self; A. Parente ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 440 KB ๐Ÿ‘ 2 views

Oligopeptides subjected to partial enzyme hydrolysis provide mixtures of sub-peptides which optimize amino acid sequence determination by fast atom bombardment mass spectrometry. The digestion mixture can be sampled directly; there is no interference from the enzyme. Complete sequences were obtained