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Model for oxygen transfer in a shake flask

✍ Scribed by J. C. Van Suijdam; N. W. F. Kossen; A. C. Joha

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
557 KB
Volume
20
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

The shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the oxygen supply is becoming a growth‐limiting factor or not. The results of measurements of the transfer coefficient of a cotton plug and the oxygen mass‐transfer coefficient k~l~a are also given.

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## Abstract Oxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of __k__~G~__a__ in shake flasks with closures

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The oxygen transfer rate (OTR) is the most suitable measurable parameter to quantify the physiological state of a culture of aerobic microorganisms since most metabolic activities depend on oxygen consumption. Online measurement of the oxygen transfer rate in stirred bioreactors is state of the art