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Mode of transmission of human T-cell leukemia virus type I (HTLV I) in a human promyelocytic leukemia HL60 cell

✍ Scribed by K. Hiramatsu; M. Masuda; H. Yoshikura

Publisher
John Wiley and Sons
Year
1986
Tongue
French
Weight
1004 KB
Volume
37
Category
Article
ISSN
0020-7136

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✦ Synopsis

HTLV-I propagated in lMR9O human diploid fibroblasts was transmitted to human myeloid leukemia HL60 cells at a low efficiency. After co-cultivation for 3 months, the viral genome was detected in 14/48 HL60 cell clones. Among the 14 HTLV-I-infected clones, 8 contained subgenomic fragments alone or in addition to the complete HTLV-I genome. The frequency of deleted proviruses (9/24 total proviruses) was unexpectedly high. Hirt's supernatant of some of the clones harboring complete HTLV-I genome@) in the chromosome contained both linear and circular HTLV-I proviral DNAs. The circular DNAs were composed of one LTR and 2 LTR closed circular proviruses. These clones produced infectious HTLV-I constitutively, which was proved by transmission of the viral genome into fresh lMR9O cells by co-cultivation. However, in these clones, re-integration of extrachromo-soma1 provirus into their own chromosomes was not observed.

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