𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Infectious transmission of human T-cell leukemia virus to rabbits

✍ Scribed by I. Miyoshi; S. Yoshimoto; I. Kubonishi; M. Fujishita; Y. Ohtsuki; M. Yamashita; K. Yamato; S. Hirose; H. Taguchi; K. Niiya; M. Kobayashi

Publisher
John Wiley and Sons
Year
1985
Tongue
French
Weight
688 KB
Volume
35
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A rabbit lymphoid cell line (Ra‐I) was established by co‐cultivation with a human T‐cell line (MT‐2) carrying human T‐cell leukemia virus (HTLV). The Ra‐l cell line is chromosomally male and is persistently infected with HTLV. Ra‐l cells, with or without mitomycin‐C treatment, were inoculated intravenously (i. v.) into 3 female rabbits. All 3 animals responded with the production of antibodies to HTLV antigens. Lymphocytes from one of these seroconverters were cultured in the presence of T‐cell growth factor (TCGF) and HTLV particles were detected in the TCGF‐grown lymphocytes which were chromosomally female. Co‐cultivation of lymphocytes from the 2 other seroconverters with lymphocytes from 2 anti‐HTLV‐negative healthy men gave rise to the establishment of an HTLV‐producing T‐cell line derived from each individual. Blood transfusion from one of the HTLV‐infected rabbits into 2 female rabbits also resulted in the seroconversion of both recipients. An HTLV‐carrying lymphoid cell line (Ra‐2) was established from one of the transfusion‐related seroconverters. The Ra‐2 cell line was initially TCGF‐dependent but later became TCGF‐independent. These results indicate that HTLV can be transmitted to rabbits. These animals may provide a suitable model system for studying the mode of transmission and pathogenicity of HTLV.

📜 SIMILAR VOLUMES

Mode of transmission of human T-cell leu
Mode of transmission of human T-cell leukemia virus type I (HTLV I) in a human promyelocytic leukemia HL60 cell
✍ K. Hiramatsu; M. Masuda; H. Yoshikura 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 French ⚖ 1004 KB

HTLV-I propagated in lMR9O human diploid fibroblasts was transmitted to human myeloid leukemia HL60 cells at a low efficiency. After co-cultivation for 3 months, the viral genome was detected in 14/48 HL60 cell clones. Among the 14 HTLV-I-infected clones, 8 contained subgenomic fragments alone or in

Significance of Postnatal Mother-to-Chil
Significance of Postnatal Mother-to-Child Transmission of Human T-lymphotropic Virus Type-I on the Development of Adult T-cell Leukemia/Lymphoma
✍ M.D. Hidenori Sugiyama; Hiroshi Doi; Kyoko Yamaguchi; Yoshiro Tsuji; Tsutomu Miy 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 489 KB 👁 1 views

In order to shed light on the mode of HTLV-I infection by mother-to-child transmission, we examined sera of school children in a highly endemic town on two separate occasions at a 6-year interval. The carrier rates in ages 15-17, 8.7 and 2.1%, were significantly higher than that in ages 6-8, 1.7 and

Human T-cell leukemia virus type I: Pseu
Human T-cell leukemia virus type I: Pseudotype neutralization of Japanese And American isolates with human and rabbit sera
✍ H. Hoshino; M. Miwa; P. R. Clapham; R. A. Weiss; I. Miyoshi; M. Yoshida 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 French ⚖ 455 KB

CCC/ZM, CCCI IOY and CCC/MT-2 cat kidney cells producing Japanese isolates of human T-cell leukemia virus type I (HTLVs) and HOSlPL human osteosarcoma cells producing an American isolate of HTLV were infected with vesicular stomatitis virus (VSV) to prepare VSV pseudotypes bearing envelope antigens