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Mixing conditions for enzyme catalysis in organic solvents

✍ Scribed by Lise Kvittingen; Birte Sjursnes; Peter Halling; Thorleif Anthonsen

Publisher
Elsevier Science
Year
1992
Tongue
French
Weight
374 KB
Volume
48
Category
Article
ISSN
0040-4020

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✦ Synopsis

When performing enzyme catalysed reactions in organic solvents the agitation method may have an influence on the result. It has been observed that stirring with magnet bars may cause a considerable decrease in the reaction rate, probably due to damage to the enzyme. This applies to both free and immobilised enzyme. However, use of a shaker or an over-head stirrer does not inactivate the enzyme. ml, i.d. 22 mm) with shaking at 130 strokes pr min (spm) or stirring with 10 or 18 mm teflon-coated magnet bars at 200-1000 'pm. The reactions performed with overhead stirrers were scaled up 10 times.

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